Apoptosis Reagents and Kits
Apexbio Technology LLC Caspase-3/7 Inhibitor I 220509-74-0 5mg
Caspase-3/7 Inhibitor I (CAS 220509-74-0) is a reversible inhibitor of caspase-3 and caspase-7 structurally belonging to the isatin sulfonamide class It exhibits selective inhibition toward caspase-3 and caspase-7 with Ki values of 60 nM and 170 nM respectively while minimally affecting caspase-9 (Ki 3 1 mM) and showing negligible activity against other caspases (caspases 1 2 4 6 and 8 Ki 25 mM) In cell-based assays this inhibitor effectively reduces apoptosis induced by camptothecin in Jurkat cells (IC50 50 M) and markedly suppresses apoptosis in chondrocytes at similar concentrations The compound is routinely utilized in apoptosis research and may offer therapeutic insights into apoptotic modulation
Sartorius Mito Membrane Potential
No wash protocol – just add and read for fast easily automated workflow.Multiplex with Qbeads PlexScreen kits for richer content.PrincipleMitochondrial membrane potential is determined by sequestration of a small fluorescent molecule inside the lumen of intact mitochondria with an active membrane potential. Upon mitochondrial depolarization the dye leaks into the cytoplasm and the cell exhibits a decrease in fluorescence.
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 4L
Duolink(R) In Situ Wash Buffers, Fluorescence | 4L
Selleck Chemical LLC ML-7 HCl 10mg 110448-33-4
ML-7 is an inhibitor of smooth muscle myosin light chain kinase (MLCK) with a Ki value of 0.3 ¿M and displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. ML-7 also inhibits PKA and PKC with Ki of 21 ?M and 42 ?M, respectively. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
Abcam Annexin V CF Blue PI Apoptosis Staining Detection Kit
Annexin V CF Blue PI Apoptosis Staining Detection Kit
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Oxaliplatin powder | 61825-94-3 | MFCD00866327 | 5mg
Oxaliplatin powder | Mol Wt: 397.29 | 61825-94-3 | MFCD00866327 | 5mg
Apexbio Technology LLC Annexin V-FITC Apoptosis Kit, 100Assays Cas No: NA MFCD: NA
Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V. There are other sizes available. Please inqury us for quote.
Apexbio Technology LLC Annexin V-Cy5 Apoptosis Kit, 100 assays. Cas: MFCD: NA. Detects Apoptosis within 10 min.
Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V.Annexin V-Cy5 Apoptosis Detection Kit uses a fluorescent conjugate of Annexin V that can easily detect PS on the cell surface after initiating apoptosis. The one-step staining procedure needs only 10 minutes. The result can be analyzed by fluorescence microscopy or by flow cytometry. Other sizes are also available. Please inqury us for quote.
Proteintech Group Inc CoraLite488 TUNEL Assay Apoptosis Detection Kit(green)
A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.
Electron Microscopy Sciences Omniprobe In-Situ Probe Tip
Custom tungsten tip with a stainless steel shank, for use with AutoProbe 300, in-situ probe tip exchange systems and Short-Cut. Tip radius is 0.5um with 8 -10 degree taper angle.This tip is also Short-Cut compatible.
Proteintech Group Inc CoraLite594 TUNEL Assay Apoptosis Detection Kit
A remarkable feature of apoptosis is the degradation of chromosome DNA, which is a common phenomenon. The degradation is very specific and regular. DNA fragments break into different lengths are of 180 bp-200 bp, showing a specific ladder like ladder pattern in agarose gel electrophoresis. The kit uses TUNEL method and uses Terminal Deoxynucleotidyl Transferase(TdT) catalyzes the incorporation of CoraLite594-dUTP into the 3´-OH terminal of the broken DNA of apoptotic cells. DNA labeled with CoraLite594- dUTP can be observed directly by fluorescence microscope or quantified by flow cytometry. TUNEL can selectively detect apoptotic cells rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug therapy.
Biotium NucView 488 Caspase 3 Assay Kit, Trial Size
NucView 488 Caspase-3 substrate is a novel, fluorogenic substrate for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrate is non-inhibitory to caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. Consisting of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety, the initially non-fluorescent, cell permeable substrate is cleaved by caspase-3 in apoptotic cells and emits green fluorescence for the detection of both caspase-3 activity and apopototic nuclear morphology, in single-cells. As apoptotic rates and expression of asociated markers varies between cells, even within the same population, single-cell detection is important. Suitable for flow cytometry, fluorescence microscopy, live cell imaging systems, or fluorescence microplate reader, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications.